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Identification and expression analysis of 11 subtilase genes during natural and induced senescence of barley plants

Colaborador(es): Roberts, Irma N. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina. E - mail: iroberts@agro.uba.ar | Veliz, Cintia G. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina | Criado, María Victoria. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina | Signorini, Ana. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina | Simonetti, Ester. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina | Caputo, Carla. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA), CONICET, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina.
ISSN: 0176-1617.Tipo de material: Artículos y capítulos. Recurso electrónico.Tema(s): BARLEY (HORDEUM VULGARE L.) | NITROGEN REMOBILIZATION | PROTEASES | PROTEOLYSIS | SENESCENCE | SUBTILASES | Recursos en línea: Haga clic para acceso en línea | LINK AL EDITOR En: Journal of Plant Physiology Vol.211, no.70 (2017), p.70-80, grafs.Resumen: Subtilases are one of the largest groups of the serine protease family and are involved in many aspectsof plant development including senescence. In wheat, previous reports demonstrate an active participa-tion of two senescence-induced subtilases, denominated P1 and P2, in nitrogen remobilization duringwhole plant senescence. The aim of the present study was to examine the participation of subtilasesin senescence-associated proteolysis of barley leaves while comparing different senescence types. Withthis purpose, subtilase enzymatic activity, immunodetection with a heterologous antiserum and geneexpression of 11 subtilase sequences identified in barley databases by homology to P1 were analyzed inbarley leaves undergoing dark-induced or natural senescence at the vegetative or reproductive growthphase. Results showed that subtilase specific activity as well as two inmunoreactive bands representing putative subtilases increased in barley leaves submitted to natural and dark-induced senescence. Gene expression analysis showed that two of the eleven subtilase genes analyzed, HvSBT3 and HvSBT6, wereup regulated in all the senescence conditions tested while HvSBT2 was expressed and up-regulated onlyduring dark-induced senescence. On the other hand, HvSBT1, HvSBT4 and HvSBT7 were down - regulatedduring senescence and two other subtilase genes (HvSBT10 and HvSBT11) showed no significant changes.The remaining subtilase genes were not detected. Results demonstrate an active participation of subti-lases in protein degradation during dark-induced and natural leaf senescence of barley plants both at thevegetative and reproductive stage, and, based on their expression profile, postulate HvSBT3 and HvSBT6as key components of senescence-associated proteolysis.
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Subtilases are one of the largest groups of the serine protease family and are involved in many aspectsof plant development including senescence. In wheat, previous reports demonstrate an active participa-tion of two senescence-induced subtilases, denominated P1 and P2, in nitrogen remobilization duringwhole plant senescence. The aim of the present study was to examine the participation of subtilasesin senescence-associated proteolysis of barley leaves while comparing different senescence types. Withthis purpose, subtilase enzymatic activity, immunodetection with a heterologous antiserum and geneexpression of 11 subtilase sequences identified in barley databases by homology to P1 were analyzed inbarley leaves undergoing dark-induced or natural senescence at the vegetative or reproductive growthphase. Results showed that subtilase specific activity as well as two inmunoreactive bands representing putative subtilases increased in barley leaves submitted to natural and dark-induced senescence. Gene expression analysis showed that two of the eleven subtilase genes analyzed, HvSBT3 and HvSBT6, wereup regulated in all the senescence conditions tested while HvSBT2 was expressed and up-regulated onlyduring dark-induced senescence. On the other hand, HvSBT1, HvSBT4 and HvSBT7 were down - regulatedduring senescence and two other subtilase genes (HvSBT10 and HvSBT11) showed no significant changes.The remaining subtilase genes were not detected. Results demonstrate an active participation of subti-lases in protein degradation during dark-induced and natural leaf senescence of barley plants both at thevegetative and reproductive stage, and, based on their expression profile, postulate HvSBT3 and HvSBT6as key components of senescence-associated proteolysis.

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