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Molecular markers to study the variability within the Eragrostis curvula complex

Colaborador(es): Zappacosta, Diego | Meier, Mauro | Carrera, Arnaldo | Pacheco, Gabriela | Cardone, Susana | Selva, Juan Pablo | Echenique, Viviana.
ISSN: 0031-9457.Otro título: Marcadores moleculares para el estudio de la variabilidad dentro del complejo Eragrostis curvula.Tipo de material: Artículos y capítulos. Recurso electrónico.Tema(s): APOMIXIS | CULTIVAR IDENTIFICATION | ERAGROSTIS | MOLECULAR MARKERS | SEED PURITY | Recursos en línea: Haga clic para acceso en línea | LINK AL EDITOR. En: Phyton Vol.80 (2011), p.211-220Resumen: Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable patterns were observed in progenies of apomictic and sexual plants, respectively. Seed purity was evaluated in seed bulks, observing a certain degree of contamination with seeds from different sources. AFLP were the markers with the highest potential for cultivar identification. The clustering using SSR and AFLP was consistent with previous studies using isozymes and morphological traits. The new tetraploid materials developed by our own group should be included within the curvula type. We also proposed the creation of a new morphological type for the diploid, because it posses morphological characteristics of robusta and molecular profiles of the conferta type.
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Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable patterns were observed in progenies of apomictic and sexual plants, respectively. Seed purity was evaluated in seed bulks, observing a certain degree of contamination with seeds from different sources. AFLP were the markers with the highest potential for cultivar identification. The clustering using SSR and AFLP was consistent with previous studies using isozymes and morphological traits. The new tetraploid materials developed by our own group should be included within the curvula type. We also proposed the creation of a new morphological type for the diploid, because it posses morphological characteristics of robusta and molecular profiles of the conferta type.

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